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tsuruhatensis has been reported to be the causative agent of catheter-related nosocomial human infections. A recently described species, closely related to Delftia acidovorans, Delftia tsuruhatensis, has been reported to cause biofouling of bioreactor membranes, reverse osmosis membrane filters and heating systems. Delftia species are widely distributed in the environment and have significant biodegradation capability. The Delftia genus resides in the Comamonadaceae family of the Betaproteobacteria class and is a Gram negative, short rod-shaped bacterium. Phage therapy has been regarded as an alternative to the need of synthesizing new antibiotics. With diminishing opportunities to discover new drugs to combat ARB infections, there is an urgent need to develop alternative therapeutic methods. ARB bacteria are becoming common, and the Centers for Disease Control and Prevention in the United States estimates more than 23,000 patients die annually due to ARB infections in the US alone. The occurrence and spread of antibiotic resistant bacteria in the environment are regarded as environmental challenges of highest concern in the twenty-first century. coli phage N4 indicating that it is a member of the N4-like phage group. Significant similarities in gene homology and order were observed between Delftia phage RG-2014 and the E. The complete genome of the isolated phage was sequenced and determined to be 73.8 kbp in length with a G + C content of 59.9%. It has an isometric icosahedrally shaped capsid with a diameter of 85 nm. The phage was found to be a dsDNA virus belonging to the Podoviridae family. RG-2014 was characterized for its type, burst size, latent and eclipse time periods of 150 ± 9 PFU/cell, 10-min, <5-min, respectively. The strain ARB-1 belongs to the Comamonadaceae family of the Betaproteobacteria class. Lytic phage RG-2014 was isolated for developing phage based therapeutic approaches against Delftia tsuruhatensis strain ARB-1. 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There’s no doubt that the effect is here to stay. Autotune has been established as a popular and creative effect. |
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